1 3

Watson Bio Lab

2.0 mL, Protein Low Binding Tube, "PROKEEP", Round Bottom "PK-20C-500" (500 tubes)

2.0 mL, Protein Low Binding Tube, "PROKEEP", Round Bottom "PK-20C-500" (500 tubes)

$82.00 USD
$82.00 USD $82.00 USD
Sale Sold out
Cat. No.:PK-20C-500
Item:2.0 mL, Protein Low Binding Tube, "PROKEEP", Round Bottom
Volume:2.0 mL
Unit:500 tubes / bag
Case:20 bags (Number of pieces in a large box for bulk purchases)

■Payment Methods Accepted:Credit Card / PayPal / Google Pay / Amazon Pay

■Delivery Fee:Free shipping fee in the U.S. - no minimum required

Contact Us View full details

About

PROKEEP - Protein Low Binding Tube

PROKEEP is a high quality tube developed for the use of protein or peptide analysis (SDS-PAGE, HPLC, MS) as it mitigates the risk of the sample loss during experiment and preservation, and improves the signals detection.

Our special blending plastic materials avoid problem caused by elution from the tube

PROKEEP tubes reduce protein binding as a result of using our special blending of raw materials with low protein/peptide absorption and our technique of high precision finishing on the mold (no surface treatment on PROKEEP). With PROKEEP, elution from the tube can be avoided and better experiment result on MALDI MS analysis and Protein Chip analysis can be anticipated (See below).

PROKEEP's Protein Low Binding performance

Loss of protein and peptide when using plastic containers is caused by the following two factors.
a. Solution remained in the container after pipetting or centrifuge.
b. Adsorption of protein and peptide to the plastic containers.
PROKEEP has been developed by approaching both of two factors.
Watson's PROKEEP containers are incomparable in terms of achieving a low residue signal.
PROKEEP's residue signal is approximately 1 / 20 million, which is 1 / 850 residue amount of other conventional hydrophilic products in the following factor (1).
No occurrence of peptide signal loss during 2 weeks-storage in the following factor (2).

（1）Residue Protein Amount: Fig 1

Test Method:
FITC-BSA solution (1 µg/mL) was put in a microtube and left for 1 hour at room temperature. The fluorescence of the surface of the tube was measured and digitized after removal of the solution, and then the residual protein mass was analyzed. This method evaluates the residual amount of protein on the surface of the tube.
Results:
The residue amount on PROKEEP low protein binding tube is extremely low, which is 1 / 20million of the protein added. Tubes with hydrophilic surface treatment exhibited higher residue amount due to characteristic of the treatment.(Fig.1)

（2）Adsorption of protein and peptide to the tube: Fig 2

Test Method:
A solution（0.1％TFA, 10 µL) equivalent to 500 fmol/µL peptide mix contained in Bruker Daltonics's Peptide calibration standard（#206195) was put in a tube and kept at 4℃ for 2 weeks. Then, the peptide in the tube was collected and analyzed by mass spectrometer（Bruker Daltonics's Ultraflex tof/ tof）.
Results:
In the case of PROKEEPs low protein binding tube, all the peaks were detected after the 2 weeks. Conventional tube's showed a part of the peaks were undetectable and peptide loss by adsorption was observed (Fig 2B).

(3) Crystallization on MALDI Target Plate

Test Method:
In the crystallization process on a target plate, which is the indispensable process of MALDI type mass spectrometer analysis, a sample collected from another company's hydrophilic treated tube exhibited a disturbance in crystallization and it was difficult to conduct an accurate measurement, and elution of resin content was suspected(Fig. 3). PROKEEPs low protein binding tube showed equivalent performance to the tube recommended by Bruker Daltonics.

Material: Virgin Polypropylene
Centrifugal force: 7,000 x G (water 1.5 mL / 15 minutes)
With scale (500 / 1000 / 1500 / 2000 µL)
Flat top (with marking space)

Locking mechanism
Safety lock system is equipped. Hard type and soft type are available.

Friendly hinge for finger
Pounded and embossed cap-edge reduces irritation to the finger, and makes it easier to close the cap.

Scale
The volume is easily recognized with the scale for each 0.5 mL described on the tube.

Contamination prevention
Lock part prevents the thumb from touching the inner cap when opening and closing.

Unique shape of cap
Easy opening cap with less force. Flat cap allows marking on the top.

Writing space
Writing space is available on the surface of tube (opposite side from the scale side).

nucleaze-free mark

Nuclease-free guaranteed
This mark shows RNase/DNase/Human-DNA free. Products with this mark are manufactured in the clean room under our high quality control, and inspected to meet our strict quality standards. A document with this mark can be issued for each lot upon your request.

  • 5 stars: 5 (71%)
  • 4 stars: 2 (29%)
  • 3 stars: 0 (0%)
  • 2 stars: 0 (0%)
  • 1 star: 0 (0%)
L
L
Good tubes

How I Use It:So I’ve been using these pcr tubes for two months mainly for my small business.Features that stood out to me:The cap works great, and I also really liked the number in the box because it simplifies my day a lot. The size was pretty good too, I tend to get more work done.My Personal Experience:If I compare this one against the others I’ve used, this one is just plain better. I wasn’t expecting to like the quality as much as I did.Pros and Cons:What was good: quality.What was not: could be cheaper.Durability:After two months, it still does its job the first time every time with no fuss. I can’t really ask for more.Final Thoughts:Overall this is best for DIY tinkerer types. It’s been good for me and my needs and I’d buy it again.

A
Amazon Customer
Good tubes with built in caps

I like that these tubes came with their own caps. Not only was this convenient but it also allowed for no cross contamination when used for testing.The tube themselves are durable and functioned well without any issues. I would say these are a good value as they worked well.

N
Nic Mc
Great for travelers with liquid supplements.

I got these for frequent travel. I have some liquid supplements, and wanted to only take what I needed rather than an entire bottle. The sides of these tubes are clearly marked by ml. They were perfect. They are tiny, easily dropped into an upcycled prescription bottle. and they seal well. No leaks. With non-staining liquid supplements, they can soak in a sink of warm soapy water and be reused with a wire brush, but the bag has plenty to replace with for my use case.

M
Marwan Gagaa
Great quality and heat resistant!

These protein low binding tubes are very good for my lab work. I got 500 pieces of 2.0 ml size. They have caps that lock well. They are natural color, can be cleaned with heat (autoclave) and have a round bottom. They are made in Japan, which I like for good quality.

A
Amazon.Customer
They don't warp after spinning at high speeds

These work as expected in our lab centrifuges without leakage and warping after spinning at excessive and high speeds.

T
T
Excellent 2 mL Eppendorf tubes

There are excellent tubes, which is exactly as I expected from this manufacturer. They close securely and are supposedly low-binding to protein. I have not personally tested this, but I do believe the company is reputable and has tested this, and if my application were particularly sensitive I have no doubt they would provide me with documentation of their analysis. What I plan to use these for is solution phase phage-display biopanning, in which phages display random peptides that bind to a target in a solution. I'm hoping the low-binding nature of the polystyrene prevents phages from sticking to it and not having access to my target.In any case, the default for this application is to use regular polystyrene tubes, so if less binding occurs with these, it will be a bonus rather than a necessity. Either way, these will work just fine.

A
A.G. Barraza
These are some great low binding tubes with locking caps.

I really like these low binding tubes with locking caps for lab work. The tubes are well made and the locking caps secure any liquid inside without leaks. Good product overall.