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Watson Bio Lab

Plasma Filter "176-600CS" (5 pieces)

Plasma Filter "176-600CS" (5 pieces)

Regular price $109.00 USD
Regular price Sale price $109.00 USD
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Cat. No.:176-600CS
Unit:5 pieces / bag
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About

For recovery of microRNA.
Separating plasma from whole blood quickly without cetrifuge.
  • For simple screening of cancer examination by microRNA.
  • For transporting microRNA with room temparature.
  • Applicable for circulating DNA.

Separating plasma from whole blood quickly without cetrifuge.

 

A special slit structure 
allows whole blood to be diffused uniformly!

A : A special slit structure allows whole blood to be diffused uniformly!
Reducing hemolysis* by plasma separation plate

*The hemolysis may affect the result of microRNA profile

B : Quick absorbing and drying plasma!
Perforated cellulose membrane for easy-to-use

 

For Quick cancer diagnosis

“For Quick cancer diagnosis”

The study of microRNA preservation on cancer patients before/after medical treatment
The graph shows the tumor-specific microRNA expression, which measured by ΔΔ CT method. The serum value after medical treatment is set as 1. The expression of microRNA which value was high before treatment decreased after. The same result was observed by the serum in the cellulose membrane of Plasma Filter.

 

■Separating plasma

Open the lid with 1 claw side

★Open the lid with 1 claw side

 

Open the lid and add 600 µL blood into the central rounded dent.
①Open the lid and add 600 µL blood into the central rounded dent.*1


*1 Assuming that 600 µL whole blood is available, it is possible to recover microRNA which quantity is equivalent of microRNA in 200 µL plasma.

 

Blood spreads, leave it for about 5 minutes.

②Blood spreads, leave it for about 5 minutes.

 

Remove the plasma separation plate, dry the separated plasma for about 1 hour on the cellulose membrane.

③Remove the plasma separation plate (A), dry the separated plasma (B) for about 1 hour on the cellulose membrane.

 

Sufficiently dried plasma is reservable*2 and transportable with the lid covered.

④Sufficiently dried plasma is reservable*2 and transportable with the lid covered.


*2 The storage period is approximately 1 to 2 weeks at room temperature depending on setting conditions.

 

■Recovery microRNA

Open the lid of 2 claws

★Open the lid of 2 claws

Easily open from the corner.
Easily open from the corner.

 

血漿が保存されているセルロースを取り外し、お好きな大きさに切ってお使いください

①By opening the lid from 2 claws side, the cellulose membrane containing plasma is easily taken off.
Cut the filter in any size along the perforation. microRNA can be extracted from the cellulose membrane by the specified elution kit.*3

*3 If applying extraction liquid slightly extra, the microRNA extraction from cellulose membrane may become improved.(miRNeasy Serum/Plasma Kit)