Preservation Plate, 96-well, Nylon "176-502C" (5 pieces)

Material: Polypropylene

Samples such as DNA can be preserved in a dry state. A new style of sample preservation.

• Space Saving
• Easy to transport and distribute
• Reduce Contamination Risk
• Recover Samples Directly on PCR Plate

Watson's Preservation Plate (Nylon type) has been developed in order to enable storage and transportation of such bioresources as nucleic acids such as DNA, RNA and oligonucleotides under room temperature and pressure.
Simply place such samples onto paper chips, then dry, the preservation is completed.

 

There is always a risk of contamination with RNase based on operator error which may influence the outcome of your experiment.
Using PVP the samples remain in the same condition for every ISH providing you have preserved samples in a suitable amount for each experiment.
The preservation period is more than 4 months under normal temperature.

 

Target DNA can be preserved and used for analysis in a later day. PVP with nylon paper chips is recommended for long chain samples like genomic DNA or high value samples. After sufficient drying, is possible to preserve samples for several months. (Preservation period may vary depending on the level of purification.)

 

■Preservation of Samples

①Check a protection seal and a plate in the package.

*Top side is where you can see paper chips.

 

②Get your sample absorbed into paper chips.
5uL can be pipetted at a time.
For bigger sample volume, divide it into several times and repeat pipetting and drying.

③Dry in room temperature.
Dry the sample for at least 1 hour. Reduced pressure drying is recommended. Keep it under 50℃ when heat drying in order to prevent devices from deteriorating. Make sure a sample to be preserved has resistance to the heat.
*Insufficient drying may result in faulty performance.

 

④Seal the plate with the protection seal.

*Make sure that the film is tightly applied. Loose sealing may cause contamination.

⑤How to preserve
Avoid high temperature, high humidity, strong lights for preservation. It is recommended to store the plate in a cool dark place with desiccants. High purity nucleic acid not contaminated by resolving enzyme etc. can be stored under room temperature. Freezing is not recommended because of such risks as contamination off sample by dew condensation, or deterioration of sample by freezing and melting. Do not store under temperature less than -40 ℃ as it may deteriorate and damage devices. Conduct half-life test as preservation time varies depending on sample's type, purity and environment.

 

■Extraction of Samples

①Peel off the protection seal on both sides.
To peel only select lines, it is easier if you cut the seal in advance using a knife or scissors.

 

②Drop a sample into tube etc.

【In case of tube】
Elute by adding eluent and mixing. In case of nucleotide, approx. 90% can be eluted in 3 minutes. Paper chips can be directly put into a container, without the step to elute a sample if it is used as primer or probe.

 

【In case of PCR plate】
Push the paper chips into wells by tweezers etc.

★The plate has a well layout that matches the standardized well position of existing 96 well plates in your lab. Simply place the plate on your 96 well plate and push the paper chip into it.

 

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