Preservation Plate, 1-well, Nylon "176-202C" (10 pieces)

Material plate：polystyrene

Samples such as DNA can be preserved in a dry state. A new style of sample preservation.

• Space Saving
• Easy to transport and distribute
• Reduce Contamination Risk
• Recover Samples Directly on PCR Plate

Watson's Preservation Plate (Nylon type) has been developed in order to enable storage and transportation of such bioresources as nucleic acids such as DNA, RNA and oligonucleotides under room temperature and pressure.
Simply place such samples onto paper chips, then dry, the preservation is completed.

 

There is always a risk of contamination with RNase based on operator error which may influence the outcome of your experiment.
Using PVP the samples remain in the same condition for every ISH providing you have preserved samples in a suitable amount for each experiment.
The preservation period is more than 4 months under normal temperature.

 

Target DNA can be preserved and used for analysis in a later day. PVP with nylon paper chips is recommended for long chain samples like genomic DNA or high value samples. After sufficient drying, is possible to preserve samples for several months. (Preservation period may vary depending on the level of purification.)

 

■Preservation of Samples

①Check a protection film and a plate in the package.

 

②Get your sample absorbed into paper chips.
Up to max. 200μL can be pipetted.

③Dry in room temperature.
Dry the sample for at least 90 mins.
Reduced pressure drying is recommended.
Keep it under 50℃ when heat drying in order to prevent devices from deteriorating.
Make sure a sample to be preserved has resistance to the heat.
*Insufficient drying may result in faulty performance.

 

③Seal with PET protection film.

*Make sure that the film is tightly applied. Loose sealing may cause contamination.

⑤How to preserve
Avoid high temperatue, high humidity, strong lights for preservation. It is recommended to store the plate in a cool dark place with desiccants. High purity nucleic acid not contaminated by resolving enzyme etc. can be stored under room temperature.
To preserve E. coli etc. alive, store the plate under 10℃. Freezing is not recommended because of such risks as contamination off sample by dew condensation, or deterioration of sample by freezing and melting. Do not store under temperature less than -40 ℃ as it may deteriorate and damage devices.
Concduct half-life test as preservation time varies depending on sample's type, purity and environment.

 

■Extraction of Samples

①Peel a PET protection film.

②Peel off the paper chip, cut it into the required size and　put it into a tube.

 

③Elute by adding eluent and mixing.
In case of nucleotide, approx. 90% can be eluted in 3 minutes.
Paper chips can be directly put into a container, without the step to elute a sample if it is used as primer or probe.

 

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