Even with perfect pipetting technique, the wrong pipette tip can sabotage your experiment.
From minor volume inconsistencies to complete sample loss, tip-related issues can create invisible errors that undermine your research.
In this article, we’ll break down common mistakes and how to avoid them.
1. Common Problems from Using the Wrong Tips
● Leakage and Volume Errors
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A loose-fitting tip allows air to enter, leading to unstable volume
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Using a non-compatible brand can damage the pipette's O-ring and compromise accuracy
● Air Bubbles in the Sample
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Poor tip shape or improper insertion causes air bubbles during aspiration
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This can ruin sensitive applications like PCR, ELISA, or qPCR
● Residual Liquid in the Tip
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Standard tips may retain viscous liquids, skewing your volume delivery
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Low-retention tips are recommended for precise measurement, especially with protein or enzymatic samples
2. Best Practices for Attaching Tips
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Insert the tip vertically
→ Tilting may prevent full sealing and cause aspirating errors -
Avoid excessive force
→ Over-insertion can damage your pipette or increase hand fatigue -
Always check tip compatibility charts
→ For example, WATSON tips are designed to fit most major pipette brands seamlessly
3. Environmental Factors That Affect Pipetting
● Temperature
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A temperature difference between the tip and liquid can cause expansion or contraction, leading to volume shifts
→ Allow both pipette tips and reagents to acclimate to room temperature before use
● Humidity
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High humidity causes moisture inside the tips, affecting measurement
→ Store tips in dry, sealed containers with desiccants
4. Tip Storage & Maintenance Tips
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Use sterile tips immediately after opening to avoid contamination or deterioration
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For bulk tips, transfer them in a clean area to racks
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Keep track of tip lot numbers and usage history in your lab notebook to trace errors if needed
Final Thoughts
Pipette tips are more than disposable plastic—they’re critical components in your experimental workflow.
Choosing the right tip and managing your technique can significantly improve accuracy, consistency, and confidence in your results.