Mastering the basics for consistent lab results
Pipettes are one of the most frequently used tools in any lab.
But even small mistakes in technique can lead to major variability in your data.
Whether you're training new team members or brushing up on fundamentals, this guide walks you through the 7 key steps of proper pipetting—and highlights the most common errors (and how to avoid them).
✅ The 7 Fundamental Steps
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Attach the tip properly
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Insert the tip vertically and press until you feel a firm click.
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Pushing too hard may damage the pipette’s internal mechanism.
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Press the plunger to the first stop
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For aspiration, press the plunger to the first resistance point (stop 1).
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Immerse the tip slightly into the liquid (~2–3 mm)
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Too deep? You risk drawing in bubbles.
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Too shallow? You may not draw the correct volume.
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Release the plunger slowly to aspirate
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Releasing too quickly can introduce air bubbles or overdraw liquid.
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Move to the dispensing location
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Avoid touching the inside wall of the receiving container with the tip.
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Dispense by pressing to the second stop
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To eject the full volume, press past the first stop to the second.
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Eject the tip using the ejector button
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Never reuse tips between samples—always use a fresh one.
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⚠️ Common Mistakes and How to Avoid Them
| Mistake | Cause | How to Fix |
|---|---|---|
| Air bubbles in the tip | Rapid aspiration or deep immersion | Aspirate slowly, dip ~2–3 mm only |
| Inconsistent volume | Unclear stop position control | Learn plunger resistance by feel |
| Loose-fitting tips | Poor attachment or incompatible tips | Use correct brand/tip and secure fit |
| Tip contacting liquid surface | Poor angle or hand positioning | Keep angle under 15°, avoid touching |
| Multiple draws for one dose | Incorrect setting or wrong volume tip | Choose proper volume range + tip size |
🎯 Pro Tips for Better Results
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Check pipette settings before each use.
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Mind the lab environment—temperature and humidity affect accuracy.
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Adjust aspiration speed for viscous or volatile liquids.
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Schedule regular calibration and keep records (especially in GMP/GLP labs).
📽️ WATSON’s Training Support(Sorry for by Japanese..)
📌 Conclusion
The phrase “practice makes perfect” rings especially true in pipetting.
A single microliter can make or break an experiment, so every detail matters.
Master the 7 steps, avoid common pitfalls, and you’ll see better, more consistent results every day.