In the world of life science research and diagnostics, precise liquid handling is the foundation of reproducible results. However, even small mistakes in pipetting and dispensing can lead to major issues in experimental data.
Here are five frequent liquid handling errors—and practical tips to avoid them.
1. Loose or Leaky Pipette Tips
Issue: A loosely attached tip can lead to leaking, inaccurate aspiration, or incomplete dispensing.
Prevention: Press the tip firmly until you hear a distinct "click." Consider using filter tips to ensure a secure fit and reduce contamination risks.
2. Air Bubble Contamination
Issue: Drawing liquid with the tip above the surface or pressing the plunger too forcefully can introduce air bubbles, affecting accuracy.
Prevention: Submerge the tip slightly below the liquid surface and operate the plunger slowly and steadily.
3. Incomplete Aspiration or Dispensing
Issue: Liquid isn’t fully aspirated or dispensed, causing significant deviation from intended volumes.
Prevention: Use the two-stop technique correctly—aspirate to the first stop and dispense all the way to the second stop to release the full volume.
4. Worn or Uncalibrated Equipment
Issue: Over time, pipette components like springs and seals degrade, leading to measurement errors.
Prevention: Schedule regular calibration (once or twice a year) and perform routine maintenance. Keep calibration certificates organized and updated.
5. Using the Wrong Tip or Pipette Size
Issue: Using a pipette that is too large or too small for the target volume can drastically reduce precision.
Prevention: Choose a pipette that covers 80–100% of the desired volume range. For example, use a 2–20 μL pipette for dispensing 20 μL.
🔧 Training and Maintenance to Avoid Trouble
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Offer hands-on training to correct bad habits and improve consistency
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Perform regular visual inspections and clean pipettes as needed
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Review storage environments—keep tips dry and dust-free
✅ Aim for Reliable Results, Every Time
Accurate liquid handling is the key to reproducible data. By addressing these common pitfalls and implementing preventative strategies, you’ll improve your workflow and confidence in your results.